Title: Webstraw: Cell Bio Anki Deck / Section 3 - Isolation and Analysis of Cell Organelles

Q: antibodies made against specific cell surface proteins can be linked to [...]
A: fluorochromes

Q: cells with bound [...] can be sorted and counted
A: antibodies

Q: [...] binds DNA in the nucleus and can show what part of the cell cycle the cells are in
A: Hoechst stain

Q: t-cells have cell surface proteins [...] and [...]
A: Thy1.2; CD3

Q: Thy1.2 binds to [...] fluorophores and CD3 binds [...] fluorophores
A: red; green

Q: [...] uses fluorescence and cell culture technologies to sort cells
A: fluorescent activated cell sorting (FACS)

Q: FACS can be used to calculate the [...] of each cell population
A: proportion

Q: cells in G2 have [...] the fluorescence intensity as cells in G1
A: twice

Q: the first overall step in isolating cell organelle is [...]
A: disruption of the cell plasma membrane

the steps in disrupting the cell membrane are
1. mechanical homogenization
2. sonication
3. pressure
4. non-ionic detergents
5. placing cells in hypotonic solution

Q: the second overall step in isolating cell organelles is [...] of the cell homogenate
A: centrifugation

Q: [...] centrifugation uses progressively increasing speeds with each speed separating the largest component
A: differential

Q: [...] centrifugation causes separation based on density
A: equilibrium density-gradient

Q: the order of organelles centrifuged is
1. [...]
2. [...], chloroplasts, lysosomes, peroxisomes
3. plasma membrane
4. [...]
A: nuclei; mitochondria; ribosomes

Q: [...] are used to separate proteins from the organelle
A: detergents

Q: [...] detergents dissolves lipid bilayers and membranes without inducing a charge
A: non-ionic

Q: [...] detergents form ions ot dissolve membranes and denatures proteins to isolate organelles
A: ionic

Q: SDS-PAGE separates proteins by [...] using [...] detergents which allow proteins to migrate through gel
A: size; ionic

Q: [...] proteins move faster towards the [...] charge in SDS-PAGE
A: smaller; negative

Q: [...] is used to detect and quantify specific proteins
A: Western Blotting (Immunoblotting)

Q: [...] is used as control in Western Blotting with resulting bands being equally dense if accurate
A: actin